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human control primary dermal fibroblast cells  (Coriell Institute for Medical Research)

 
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    Coriell Institute for Medical Research human control primary dermal fibroblast cells
    Human Control Primary Dermal Fibroblast Cells, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+control+primary+dermal+fibroblast+cells/human+fibroblasts/pm39456243-65-0-10
    Average 90 stars, based on 1 article reviews
    human control primary dermal fibroblast cells - by Bioz Stars, 2026-11
    90/100 stars

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    Control:

    Article Title: Enhancing Cellular Homeostasis: Targeted Botanical Compounds Boost Cellular Health Functions in Normal and Premature Aging Fibroblasts.
    Article Snippet: .. Human control primary dermal fibroblast cells were sourced from the Coriell Institute for Medical Research (Camden, NJ, USA), while HGPS cells were acquired from the Progeria Research Foundation Cell and Tissue Bank. .. The primary fibroblast cultures were maintained in a cell incubator (Binder, Tuttlingen, Germany, 9140-0046) in DMEM (Thermo Fisher—Gibco, Waltham, MA, USA, D6429) supplemented with 15% fetal bovine serum (FBS; Thermo Fisher—Gibco, Waltham, MA, USA, 10270106), 1% L-glutamine (Thermo Fisher—Gibco, Waltham, MA, USA, 25030081), 1% gentamycin (Thermo Fisher—Gibco, Waltham, MA, USA, 15710049), and 1% penicillin/streptomycin (Thermo Fisher—Gibco, Waltham, MA, USA, 1514022) under a 5% CO2 atmosphere and 37 ◦C.



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    Mitochondrial respiration is impaired in fibroblasts derived from patients with DLD deficiency. Mitochondrial oxygen consumption was assessed in controls (Ctrl1 and Ctrl2) and patient (Pt1–Pt6) fibroblasts using high-resolution respirometry (Oroboros O2k). ( A ) Routine respiration; ( B ) maximal respiration calculated as the difference between FCCP-stimulated and α-chaconine–permeabilized rates; ( C ) complex I-linked respiration (NADH-linked respiration, N-pathway), calculated as the difference between ADP and α-chaconine; ( D ) complex II-linked respiration (NS-pathway) calculated as the difference between respiration after rotenone and α-chaconine addition; ( E ) effect of complex I inhibition, calculated as the difference between FCCP-stimulated and rotenone-inhibited respiration; and ( F ) complex I/complex II respiration ratio (complex I-linked activity divided by complex II-linked activity). Each open circle represents an independent experimental run (N = 4–8 repeats per sample). All data were normalized to cell number. Statistical analysis was performed using the Mann–Whitney U test. * p < 0.05 vs. Ctrl1; numerical p values (0.05 < p < 0.1) are indicated on the plots. Abbreviations: Ctrl, control; Pt, patient.

    Journal: Antioxidants

    Article Title: Bioenergetic Signatures of DLD Deficiency: Dissecting PDHc- and α-KGDHc-Linked Defects

    doi: 10.3390/antiox15010019

    Figure Lengend Snippet: Mitochondrial respiration is impaired in fibroblasts derived from patients with DLD deficiency. Mitochondrial oxygen consumption was assessed in controls (Ctrl1 and Ctrl2) and patient (Pt1–Pt6) fibroblasts using high-resolution respirometry (Oroboros O2k). ( A ) Routine respiration; ( B ) maximal respiration calculated as the difference between FCCP-stimulated and α-chaconine–permeabilized rates; ( C ) complex I-linked respiration (NADH-linked respiration, N-pathway), calculated as the difference between ADP and α-chaconine; ( D ) complex II-linked respiration (NS-pathway) calculated as the difference between respiration after rotenone and α-chaconine addition; ( E ) effect of complex I inhibition, calculated as the difference between FCCP-stimulated and rotenone-inhibited respiration; and ( F ) complex I/complex II respiration ratio (complex I-linked activity divided by complex II-linked activity). Each open circle represents an independent experimental run (N = 4–8 repeats per sample). All data were normalized to cell number. Statistical analysis was performed using the Mann–Whitney U test. * p < 0.05 vs. Ctrl1; numerical p values (0.05 < p < 0.1) are indicated on the plots. Abbreviations: Ctrl, control; Pt, patient.

    Article Snippet: Dermal fibroblast primary cell lines from six genetically confirmed patients with DLD deficiency were obtained from the Pediatric Metabolic Disease Unit, Sheba Medical Center (IRB# SMC-21-8644, Figure 1, Table 1, and ), as well as two control cell lines: a control human dermal fibroblast cell line was purchased from ATCC (PCS-201-012; Ctrl 1, Manassas, VA, USA), and a primary cell line from a 39-year-old healthy male (Ctrl 2).

    Techniques: Derivative Assay, Inhibition, Activity Assay, MANN-WHITNEY, Control

    Figure 4. In vitro combined cytotoxic effects of βCD, CUR, and βCD-CUR. Normalized cell viability (%) after 24 h treatment against (a) SW480, (b) HCT116, and (c) NHDF cells. Data are shown as mean ± S.D. of three independent replicates, where statistically significant differences between CUR values versus βCD-CUR values are denoted as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).

    Journal: International journal of molecular sciences

    Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.

    doi: 10.3390/ijms232112866

    Figure Lengend Snippet: Figure 4. In vitro combined cytotoxic effects of βCD, CUR, and βCD-CUR. Normalized cell viability (%) after 24 h treatment against (a) SW480, (b) HCT116, and (c) NHDF cells. Data are shown as mean ± S.D. of three independent replicates, where statistically significant differences between CUR values versus βCD-CUR values are denoted as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).

    Article Snippet: Human CRC cell lines HCT116 (ATCC®® CCL-247TM) and SW480 (ATCC®® CCL-228TM), as well as normal human cell control NHDF (ATCC®® PCS-201-012TM), were cultured in the media containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 100 U/mL penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Inc., USA) and 10.0% (v/v) fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., USA).

    Techniques: In Vitro